TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX reference dye*

Ordering Information:

Cat. 17272 1ml

Cat. 17273 5ml

Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*.

Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
TAQuest™ qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. The master mix includes our proprietary TAQuest™ Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer. You only need to add template and target primers to run the desired PCR reactions. The Hot Start Taq DNA polymerase allows you to set up a PCR reaction at room temperature, thus minimizing non-specific product formation.
In combination with an optimized buffer, the enzyme ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. The Helixyte Green intercalating dye allows rapid DNA detection and analysis without using sequence-specific probes. This master mix contains a low amount of ROX reference dye.
Platform: qPCR
Instrument specification:  SYBR Green filter