TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*.

Research Use Only (RUO)
Freeze (< -15 °C); Minimize light exposure
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. The master mix delivers results within 50 minutes for 40 cycles of PCR in a 20 uL reaction volume. The mix includes an optimized buffer containing dNTPs and our proprietary TAQuest™ FAST Hot Start Taq DNA Polymerase enzyme, an enzyme designed to allow instant hot start which minimizes non-specific product formation thus allowing room temperature reaction setup. Only template and target primers are required to run the desired PCR reactions.
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. The Helixyte Green intercalating dye allows rapid DNA detection and analysis without using sequence-specific probes. This master mix contains a low amount of ROX reference dye.
Platform: qPCR
Instrument specification:	SYBR Green filter

Ordering Information

Cat. 17278 1ml

Cat. 17279 5ml