iFluor® 546 Styramide

Platform : Fluorescent Microscope

Excitation: Cy3/TRITC filter set,  Emission: Cy3/TRITC filter set,   Recommended plate:  Black wall/clear bottom

Cat. 45025 100 Slides

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 546 Styramide is a superior replacement for Alexa Fluor 546 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Spectral properties

Correction Factor (260 nm):   0.25                   Correction Factor (280 nm):   0.15                    Extinction coefficient (cm -1 M -1):    100000 Excitation (nm):     541                                             Emission (nm):       557                                           Quantum yield:      0.67