iFluor® 450 succinimidyl ester

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 450 dyes have fluorescence excitation and emission maxima of ~451 nm and ~502 nm respectively. These spectral characteristics make them an excellent choice for multicolor applications. iFluor® 450 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. iFluor® 450 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for fluorescence imaging and flow cytometric applications. iFluor® 450 dye conjugates are bright, providing an additional color between Alexa Fluor® 430 and 488 (ThermoFisher).

Cat. 1026 1mg

Cat. 71026 100ug

Cat. 71506 5mg

Cat. 71556 10mg

Spectral properties

Correction Factor (260 nm): 0.45 Correction Factor (280 nm): 0.27 Extinction coefficient (cm -1 M -1) : 40000 Excitation (nm): 451 Emission (nm): 502 Quantum yield: 0.821