iFluor® 350 Styramide

Fluorescence images of HeLa cells labeled with rabbit anti-Tubulin primary antibody. Cells were then stained with a HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 350 Styramide™ (Left) or Alexa Fluor® 350 tyramide (Middle and Right), respectively. Fluorescence images were taken using the DAPI filter set and the exposure time was labeled accordingly. iFluor® 350 Styramide™ shows significantly higher fluorescence intensity than Alexa Fluor® 350 tyramide if under the same exposure time (25 ms). Alexa Fluor® 350 tyramide requires exposure time higher (125 ms) to visualize the staining.

Cat. 45000 100 Slides

Platform : Fluorescence Microscope

Excitation: DAPI filter set, Emission: DAPI filter set, Recommended plate: Black wall/clear bottom

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 350 Styramide is a superior replacement for Alexa Fluor 350 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Spectral properties
Correction Factor (260 nm): 	0.83
Correction Factor (280 nm):	0.23
Extinction coefficient (cm -1 M -1):	20000
Excitation (nm):	345
Emission (nm):    	450
Quantum yield: 	0.95